%0 Journal Article
%T Label-free and real-time detection of interactional dynamic processes of rabbit IgG with different concentrations and goat anti-rabbit IgG by oblique-incidence reflectivity difference method<br>用斜入射光反射差法无标记实时监测不同浓度兔IgG和山羊抗兔IgG反应的动力学过程
%A He Li-Ping
%A Sun Yue
%A Dai Jun
%A Wang Jing-Yi
%A L&
%A Hui-Bin
%A Wang Shu-Fang
%A Jin Kui-Juan
%A Zhou Yue-Liang
%A Yang Guo-Zhen
%A <br>何立平
%A 孙悦
%A 戴俊
%A 王京义
%A 吕惠宾
%A 王淑芳
%A 金奎娟
%A 周岳亮
%A 杨国桢
%J 物理学报
%D 2012
%I 
%X We successfully label-free and real-time detecte the interactional dynamic processes of rabbit IgG with different concentrations (1.25 mg/mL, 2.50 mg/mL and 5.00 mg/mL), and goat anti-rabbit IgG with a concentration of 0.02 mg/mL by the oblique-incidence reflectivity difference method (OIRD). The dynamic curves and times of interaction, response to concentration of rabbit IgG, are obtained. The experimental results demonstrate that the OIRD method can not only can distinguish the biomolecular interactions, but also real-time detect the interactional dynamic processes of biomolecules, indicating that the OIRD method has promising and extensive applications for the investigating of biomolecular interactions.
%K oblique-incidence reflectivity difference method
%K label-free and real-time detection
%K interaction dynamic process of proteins<br>斜入射光反射差法
%K 无标记和实时监测
%K 反应动力学过程
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=47EA7CFDDEBB28E0&jid=29DF2CB55EF687E7EFA80DFD4B978260&aid=790A9358A1C1D655ED9025D9033506F6&yid=99E9153A83D4CB11&vid=1D0FA33DA02ABACD&iid=B31275AF3241DB2D&sid=BDF82F6D5C224D7F&eid=BDF82F6D5C224D7F&journal_id=1000-3290&journal_name=物理学报&referenced_num=0&reference_num=19