%0 Journal Article
%T CD40-Activated B Cells Can Efficiently Prime Antigen-Specific Na£¿ve CD8+ T Cells to Generate Effector but Not Memory T cells
%A M¨¦lissa Mathieu
%A Natacha Cotta-Grand
%A Jean-Fran£¿ois Daudelin
%A Salix Boulet
%A R¨¦jean Lapointe
%A Nathalie Labrecque
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0030139
%X Background The identification of the signals that should be provided by antigen-presenting cells (APCs) to induce a CD8+ T cell response in vivo is essential to improve vaccination strategies using antigen-loaded APCs. Although dendritic cells have been extensively studied, the ability of other APC types, such as B cells, to induce a CD8+ T cell response have not been thoroughly evaluated. Methodology/Principal Findings In this manuscript, we have characterized the ability of CD40-activated B cells, stimulated or not with Toll-like receptor (TLR) agonists (CpG or lipopolysaccharide) to induce the response of mouse na£¿ve CD8+ T cells in vivo. Our results show that CD40-activated B cells can directly present antigen to na£¿ve CD8+ T cells to induce the generation of potent effectors able to secrete cytokines, kill target cells and control a Listeria monocytogenes infection. However, CD40-activated B cell immunization did not lead to the proper formation of CD8+ memory T cells and further maturation of CD40-activated B cells with TLR agonists did not promote the development of CD8+ memory T cells. Our results also suggest that inefficient generation of CD8+ memory T cells with CD40-activated B cell immunization is a consequence of reduced Bcl-6 expression by effectors and enhanced contraction of the CD8+ T cell response. Conclusions Understanding why CD40-activated B cell immunization is defective for the generation of memory T cells and gaining new insights about signals that should be provided by APCs are key steps before translating the use of CD40-B cell for therapeutic vaccination.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0030139