%0 Journal Article
%T The Translation Elongation Factor eEF-1B¦Â1 Is Involved in Cell Wall Biosynthesis and Plant Development in Arabidopsis thaliana
%A Zakir Hossain
%A Lisa Amyot
%A Brian McGarvey
%A Margaret Gruber
%A Jinwook Jung
%A Abdelali Hannoufa
%J PLOS ONE
%D 2012
%I Public Library of Science (PLoS)
%R 10.1371/journal.pone.0030425
%X The eukaryotic translation elongation factor eEF-1B¦Â1 (EF1B¦Â) is a guanine nucleotide exchange factor that plays an important role in translation elongation. In this study, we show that the EF1B¦Â protein is localized in the plasma membrane and cytoplasm, and that the transcripts should be expressed in most tissue types in seedlings. Sectioning of the inflorescence stem revealed that EF1B¦Â predominantly localizes to the xylem vessels and in the interfascicular cambium. EF1B¦Â gene silencing in ef¦Â caused a dwarf phenotype with 38% and 20% reduction in total lignin and crystalline cellulose, respectively. This loss-of-function mutant also had a lower S/G lignin monomer ratio relative to wild type plants, but no changes were detected in a gain-of-function mutant transformed with the EF1B¦Â gene. Histochemical analysis showed a reduced vascular apparatus, including smaller xylem vessels in the inflorescence stem of the loss-of-function mutant. Over-expression of EF1B¦Â in an eli1 mutant background restored a WT phenotype and abolished ectopic lignin deposition as well as cell expansion defects in the mutant. Taken together, these data strongly suggest a role for EF1B¦Â in plant development and cell wall formation in Arabidopsis.
%U http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0030425