%0 Journal Article
%T The application of total internal reflection fluorescence microscopy in single fluorophore molecules axial imaging<br>全内反射荧光显微术应用于单分子荧光的纵向成像
%A 王琛
%A 王桂英
%A 徐至展
%J 物理学报
%D 2004
%I 
%X Here we present a method of modeling fluorophore molecules as spheres in evanescent fields mathematically instead of the assumption of top-hat function distribution. Based on this model, we discuss that it is possible to calculate relative separation distances of the molecules, and their axial positions and diameters with the total internal reflection fluorescence microscopy(TIRFM) due to its unique intensity pattern in the z direction. In the whole process, we need merely to change the incident angle once, that is to get only two frame imaging of TIRFM.
%K total internal reflection fluorescence microscopy
%K axial distribution reconstruction<br>全内反射荧光显微术，
%K 纵向重构
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=6E709DC38FA1D09A4B578DD0906875B5B44D4D294832BB8E&cid=47EA7CFDDEBB28E0&jid=29DF2CB55EF687E7EFA80DFD4B978260&aid=1C0C8EF65FB059AD&yid=D0E58B75BFD8E51C&vid=8E6AB9C3EBAAE921&iid=94C357A881DFC066&sid=E84660E787B699A9&eid=DF8B97D5075E2D12&journal_id=1000-3290&journal_name=物理学报&referenced_num=4&reference_num=12