%0 Journal Article %T A new method of microbial prospecting for oil and gas based on BIOLOG metabolic fingerprinting analysis
一种基于BIOLOG代谢指纹分析的油气微生物勘探新方法 %A Liao Xianyan %A Zhang Tao %A Zeng Jun %A Sun Jian %A Hu Wen %A Lou Kai %A
Liao X %A Zhang T %A Zeng J %A Sun J %A Hu W %A Lou K %J 微生物学报 %D 2012 %I %X Objective]Establishing a new method of microbial prospecting for oil and gas based on microbial community metabolic function.Methods]In this study,45 shallow reservoir soil samples and 25 non-reservoir soil samples were collected.Carbon catabolic activity of the microbial community was measured by biolog microplate,and microbial anomaly of reservoir was judged by discriminant function.Result] We screened 10 typical carbon sources of 95 carbon sources which could reflect the differences of reservoir and non-reservoir soil microbial community structure and made the validation test set to experimental zone and controlled zone samples using the discriminant function.The discriminate rate of reservoir and non-reservoir were 97.8% and 100% respectively and the overall accuracy was 98.6%.Conclusion] Biolog technology provided a highly efficient and precise method for preliminarily predicting oil and gas reservoir. %K soil microbial community %K oil and gas exploration %K biolog technology
土壤微生物群落 %K 油气勘探 %K BIOLOG技术 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9FDF75195748F95D0069402AA1EB3C79&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=E158A972A605785F&sid=42D7028D961473F8&eid=B99A53AADE50D922&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0