%0 Journal Article
%T Construction of ompW knock-out mutants of Escherichia coli to increase sensitivity to neomycinsulphate and ampicillin<br>大肠杆菌外膜蛋白ompW基因敲除菌的构建及其对两种抗生素的敏感性
%A Xianbin Wu
%A Haijie Zou
%A Lihua Tian
%A Jianyi Pan
%A Fukun Zhao
%A <br>吴贤斌
%A 邹海杰
%A 田丽花
%A 潘建义
%A 赵辅昆
%J 微生物学报
%D 2012
%I 
%X Objective]To investigate the contribution of an outer membrane protein OmpW to tolerance neomycinsulphate and ampicillin of Escherichia coli K12.Methods] The ompW knock-out mutant(ΔompW) of E.coli K12 was generated using λ-Red recombination system.Then the minimal inhibitory concentration(MIC) and the survival rates under 1/2 MIC of neomycinsulphate or ampicillin of ΔompW and E.coli K12 were determined respectively.Results] The ΔompW was successfully obtained through confirmation of PCR analysis at the gene level and Western blot analysis at the protein level.The MIC of neomycinsulphate of ΔompW is 1.7 μg/mL.The value is much lower than that of E.coli K12,which is 8.0 μg/mL.Difference of survival rates under 1/2 MIC of neomycinsulphate of ΔompW and E.coli K12 was also observed,and their survival rates are 39% and 98%,respectively.The MIC of ampicillin of ΔompW is 3.3 μg/mL.The value is also lower than that of E.coli K12(16.0 μg/mL).The survival rates under 1/2 MIC ampicillin of ΔompW and E.coli K12 are 30.3% and 70.38%,respectively.Conclusion] The ΔompW is much more sensitive to neomycinsulphate and ampicillin than its parent strain.The result indicated that OmpW played crucial role in bacteria resistance of drug.
%K Red recombination
%K outer membrane protein
%K OmpW
%K MIC
%K survival rate<br>Red同源重组
%K 外膜蛋白
%K OmpW
%K 最小抑菌浓度
%K 生存率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=1D086AF23E396C1EE9C96F78A8F8D229&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=5D311CA918CA9A03&sid=5D5B6851F9BAEE73&eid=8E4B4A74C5BF6530&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0