%0 Journal Article
%T Characterization of salicylate 5-hydroxylase for phenanthrene degradation using moderately halophilic Martelella sp. AD-3<br>中度嗜盐菌Martelella sp. AD-3 降解菲过程中水杨酸-5-羟化酶的酶学性质
%A Fei Dong
%A Changzheng Cui
%A Tiancai Feng
%A Yaoyu Feng
%A Yongdi Liu
%A <br>董斐
%A 崔长征
%A 冯天才
%A 冯耀宇
%A 刘勇弟
%J 微生物学报
%D 2012
%I 
%X Objective] To identify the relationship between salicylate 5-hydroxylase activity and the degradation rate of phenanthrene by moderately halophilic Martelella sp.AD-3,and to investigate their enzymatic characteristics.Methods] The products resulted from degradation of salicylic acid using the crude enzyme produced by AD-3 was analyzed using HPLC analysis,and salicylate 5-hydroxylase activities at different conditions was calculated according to the change of NADH absorbance at 340 nm.Results] The salicylate 5-hydroxylase activity was higher during log-phase and early stationary phase of AD-3,and the enzyme activity was in consistent with phenanthrene degradation rate.The enzyme production was induced by phenanthrene and salicylic acid.The highest enzyme activity,132.8 nmol/(min·mg),from strain AD-3 was obtained under the phenanthrene concentration of 200 mg/L,3% salinity and a pH of 9.0.The optimum conditions for degradation of salicylic acid using this enzyme were 30℃,pH 7.5,and salinity 3%.The Vmax and Km for the enzyme were 200 nmol/(min·mg) and 8.7 μmol/L,respectively.Conclusion] The salicylate 5-hydroxylase was produced in the degradation of phenanthrene in AD-3.The enzyme activity was related to phenanthrene degradation rate.
%K phenanthrene
%K salicylate 5-hydroxylase
%K enzyme activity
%K salinity<br>菲
%K 水杨酸-5-羟化酶
%K 酶活
%K 盐度
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=1D086AF23E396C1ECE540E28C0F7D1C5&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=5D311CA918CA9A03&sid=80B081C203919926&eid=3A834AEE2C42102E&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0