%0 Journal Article
%T PB1-F2 protein of influenza A virus interacts with human MOAP-1 protein<br>A型流感病毒PB1-F2蛋白与MOAP-1的相互作用
%A Yuming Cui
%A Peili Hou
%A Maolin Zhang
%A Ming Duan
%A Zhenhong Guan
%A <br>崔雨明
%A 侯佩莉
%A 张茂林
%A 段铭
%A 关振宏
%J 微生物学报
%D 2012
%I 
%X Objective] To identify the interaction between influenza A virus PB1-F2 and human modulator of apoptosis 1(MOAP-1).Methods] The recombinant plasmid pACT2-MOAP-1 was constructed and co-transformed into yeast AHl09 with pGBKT7-PB1-F2.The growth of the co-transformants on quadruple dropout medium and β-galactosidase activity of the reporter gene were tested.We further confirmed the interaction of cellular protein MOAP-1 and PB1-F2 by glutathione S-transferase(GST)pull-down and co-immunoprecipitation(Co-IP) assays.In addition,we investigated the effect of PB1-F2 on MOAP-1 protein level by Western blot.Results] The results of yeast two-hybrid assay showed that MOAP-1 specifically interacted with PB1-F2 in yeast cells.Furthermore,the binding of MOAP-1 with PB1-F2 was demonstrated by glutathione S-transferase pull-down and Co-IP assays.PB1-F2 could upregulate exogenous MOAP-1 protein level.Conclusion] These results suggested that influenza virus PB1-F2 interacted with MOAP-1 and it might be involved in the regulation of cell growth and apoptosis via association with MOAP-1.
%K Influenza A virus
%K PB1-F2
%K MOAP-1
%K protein-protein interaction<br>A型流感病毒
%K PB1-F2
%K MOAP-1
%K 蛋白相互作用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=2350B5576F89D0720D02EFE6D45BFFAC&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=9CF7A0430CBB2DFD&sid=7D34DED3F877BD2D&eid=A91C28383511878F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0