%0 Journal Article
%T Three successive extractions reduce quantification bias of microbial communities associated with incomplete DNA recovery in soil<br>3 次连续重复提取DNA 能较好反映土壤微生物丰度
%A Yun Guo
%A Yucheng Wu
%A Xiangui Lin
%A Wenhui Zhong
%A Weixin Ding
%A Jianguo Zhu
%A Zhongjun Jia
%A <br>郭赟
%A 吴宇澄
%A 林先贵
%A 钟文辉
%A 丁维新
%A 朱建国
%A 贾仲君
%J 微生物学报
%D 2012
%I 
%X Objective] To assess the quantification bias associated with incomplete extractions of soil microbial DNA and the feasibility of air-dried soil for microbial ecology study.Methods] The flooded rice soil and upland wheat soil were used,and multiple extractions of soil microbial DNA was performed by lysing a single sample for 5 successive times.The copy number of 16S rRNA and amoA genes of Archaea and Bacteria was quantified in each DNA extraction by real-time PCR.Results] Cumulative DNA yields in 3 successive extractions accounted for more than 76% of microbial DNA in soils,and more than 77.5% of gene copies could be recovered.Air-drying decreased the abundance of bacterial 16S rRNA gene and archaeal 16S rRNA gene by 90.3% and 12.5%,and the abundance of bacterial and archaeal amoA genes showed a decline by 81.2% and 84.3%,respectively.The decline showed similar trend in two soils,suggesting air-dried soil could be of choice for biogeographic survey of microbial communities.Conclusion] Three successive extractions of microbial DNA in soil could be of choice for microbial ecology study in order to reduce quantification bias associated with incomplete DNA recovery.Air-dried soil could be employed under certain circumstances,and further investigation is warranted for the underlying mechanism by which microbial communities manage to survive the desiccation of soil.
%K successive extractions of DNA in soil
%K air-dry treatment
%K archaea
%K bacteria
%K ammonia oxidizing archaea
%K ammonia oxidizing bacteria<br>土壤DNA连续提取
%K 风干处理
%K 古菌
%K 细菌
%K 氨氧化古菌
%K 氨氧化细菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=8324B84C7395C65674FC2788032217B7&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=DF92D298D3FF1E6E&sid=117143DBED3B4430&eid=E23857687CD2EE51&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0