%0 Journal Article
%T Recombinant porcine lung surfactant protein A inhibits porcine reproductive and respiratory syndrome virus infection into host cells in vitro<br>重组猪肺表面活性蛋白A在体外可抑制PRRSV感染宿主细胞
%A Feng Zhang
%A Fei Zhong
%A Xiujin Li
%A Xingxing Wang
%A Kao Zhang
%A Huihui Chen
%A Zhen Li
%A Wenyan Li
%A Hongli Pan
%A Dongmei Han
%A <br>张峰
%A 仲飞
%A 李秀锦
%A 王幸兴
%A 张考
%A 陈慧慧
%A 李振
%A 李文艳
%A 潘红丽
%A 韩冬梅
%J 微生物学报
%D 2012
%I 
%X Objective] To investigate the antiviral activity of porcine lung surfactant protein A(SP-A) to porcine reproductive and respiratory syndrome virus(PRRSV) in vitro.Methods] The SP-A gene was amplified by PCR from the plasmid containing porcine SP-A gene,and subcloned into pcDNA3.1A-CD5 vector containing the human CD5 signal peptide to generate SP-A eukaryotic expression vector pcDNA-CD5-SPA/MH.The recombinant expression vector was transfected into HEK293T cells mediated with calcium phosphate.The expressed recombinant SP-A was identified by Western blot and purified from culture medium by Ni-NTA-Agarose beads.The binding activity of SP-A with PRRSV was identified by ELISA.The antiviral activity of SP-A to PRRSV was analyzed by viral titer reduction assays on MARC-145 cells and porcine alveolar macrophages(PAM).Results] The results showed that the eukaryotic expression vector of SP-A gene could mediate SP-A expression in HEK293T cells,the expressed SP-A could bind PRRSV in a dose dependent manner.The PRRSV incubated in advance with SP-A showed the lower infective activity compared with no-SP-A-incubated PRRSV on both MARC-145 cells and porcine alveolar macrophages.The SP-A-treated PRRSV titers in MARC-145 cells and PAM cells were significantly lower than that of SP-A-untreated PRRSV at 72 h post-infection.Conclusion] Recombinant porcine SP-A significantly inhibit the infection of PRRSV to the host cells in vitro,which indicates that recombinant SP-A possesses anti-PRRSV activity.
%K recombinant porcine lung surfactant protein A
%K antiviral activity
%K porcine reproductive and respiratory syndrome virus
%K eukaryotic expression<br>猪肺表面活性蛋白A
%K 抗病毒活性
%K 猪繁殖与呼吸综合征病毒
%K 真核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=FDB09CD97DEFC4B6CC70DB8DF2C62303&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=94C357A881DFC066&sid=BBA8B1249CDAA6CE&eid=36C49E1242CC2C7A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0