%0 Journal Article
%T Biologic Characteristics of Eight Podoviridae Stx2-converting phage<br>8株动物源编码Stx2短尾噬菌体的生物学特性
%A Luming Xi
%A Liangke Su
%A Jianhe Sun
%A Yaxian Yan
%A <br>夏炉明
%A 苏良科
%A 孙建和
%A 严亚贤
%J 微生物学报
%D 2008
%I 
%X Objective] We studied biologic characteristics of Stx2-converting phage induced from Escherichia coli O157 by mitomycin C. Methods] Eight Stx2-converting phages were isolated from E. coli O157 and identified by PCR. The phage particles were purified and phage DNA was extracted. Random priming digoxin (DIG)-labeled stx2-specific gene probe was prepared for Southern blot. The morphology of these phages were studied by electron microscopy. Protein profiles were analyzed by Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis (SDS-PAGE). Restriction fragment length polymorphism (RFLP) was used to confirm the size, type, and polymorphism of the purified phage genome. Results] These 8 phages were confirmed Stx2-converting phage and DIG-labeled probe was highly specific. All phages had a regular hexagonal head and a short tail, belonging to Podoviridae phage families. The Stx2 phages had genome sizes in the range of 48 to 65.3kb, consisting of dou-ble-stranded DNA. The restriction fragment length polymorphism of these phages showed different groups, although the SDS-PAGE protein profiles of these phages were similar. Conclusion] These 8 Stx2-converting phages with similar morphol-ogy belonged to Podoviridae phage families. The protein profiles were highly identical. We could differentiate these Stx2-converting phages according to their restriction fragment length polymorphism patterns.
%K SDS-PAGE<br>Stx2噬菌体
%K 形态特征
%K 酶切多态性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=9095B85E015102E4128075F341AFFE13&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=5D311CA918CA9A03&sid=F88EF6DD822B0FF5&eid=D3CBA54FACB30BD9&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=12