%0 Journal Article
%T Storage of Oenococcus oeni in liquid nitrogen<br>酒酒球菌液氮超低温保存
%A Liye Du
%A Hua Wang
%A Gang Jin
%A Cuixia Li
%A Hua Li
%A <br>杜立业
%A 王华
%A 金刚
%A 李翠霞
%A 李华
%J 微生物学报
%D 2011
%I 
%X Objective]To develop cryopreservation for Oenococcus oeni.Methods] We investigated the influence of the age and concentration of the strain in the protective medium,rate of freezing,temperature of thawing and the protective medium on the number of viable cells after cryopreservation by colony counting.Results] We got the highest number of viable cells after cryopreservation by collecting cells in the early stationary growth phase,keeping their concentration in the protective medium at 109 CFU/mL,transfer...
%K Oenococcus oeni
%K liquid nitrogen cryopreservation
%K viable cells after cryopreservation<br>酒酒球菌
%K 液氮超低温保存
%K 冷冻存活率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D1920AB56F28E37C062B82FA3B355297&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=9CF7A0430CBB2DFD&sid=08DDB398556FE547&eid=3ED1EAB217774597&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=25