%0 Journal Article
%T Composition diversity of lactic acid bacteria (LAB) community Al2 used for alfalfa silage<br>苜蓿青贮用乳酸菌复合系Al2的组成多样性
%A WANG Xiao-fen
%A GAO Li-juan
%A YANG Hong-yan
%A WANG Wei-dong
%A CUI Zong-jun
%A <br>王小芬
%A 高丽娟
%A 杨洪岩
%A 王伟东
%A 崔宗均
%J 微生物学报
%D 2006
%I 
%X Alfalfa is the most important forage grass that is difficult to ensile for good quality.Using silage inoculants are the important way for preservation of alfalfa silage.Through continuous restricted subcultivation,a lactic acid bacteria(LAB) community Al2 was selected from well-fermented alfalfa silage.Plate isolation and Denaturing Gradient Gel Electrophoresis(DGGE),construction of 16S rDNA clone library were used to identify the composition diversity of Al2 community,with 7 strains detected,and they were all belonged to Lactobacillus.The composition ratios of the 7 strains were 55.21%,19.79%,14.58%,3.13%,3.13%,3.13%,1.03% according to 16S rDNA clone library.Al2-1i,Al2-2i,Al2-3i,corresponding to L.plantarum(99.9%),L.kimchii(99.4%),L.farciminis(100%) were detected by plate isolation.Among 3 isolates,Al2-1i had the highest ability of dropping pH and producing lactic acid,and the amount of lactic acid was reach to 18g/L at 24h cultivated in MRS media.The ability of dropping pH and producing lactic acid of Al2-3i was the lowest.From DGGE profiles,the dominant strains in Al2 community were L.plantarum and L.kimchii.L.plantarum was detected during the whole process,and L.kimchii was detected in the later phase.
%K Lactic acid bacteria(LAB)
%K Community
%K DGGE
%K 16S rDNA clone library
%K Composition diversity<br>乳酸菌
%K 复合菌系
%K DGGE
%K 16S
%K rDNA
%K 克隆文库
%K 多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=48CF7B403384A7B2&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=94C357A881DFC066&sid=70081086C74F4110&eid=FAC9AF09A23B46DD&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=19