%0 Journal Article
%T Denaturing high-performance liquid chromatography for identifying four categories of diarrheagenic Escherichia coli<br>变性高效液相色谱检测食品中致泻性大肠杆菌
%A Junyi Xu
%A Jijuan Cao
%A Qiuyue Zheng
%A Xin Zhao
%A Pingping Yan
%A <br>徐君怡
%A 曹际娟
%A 郑秋月
%A 赵昕
%A 闫平平
%J 微生物学报
%D 2008
%I 
%X Abstract: Objective] We report a new molecular technique for the analysis of four categories of diarrheagenic Es-cherichia coli based on the separation of PCR-amplified target fragments by denaturing high-performance liquid chroma-tography (DHPLC). Methods] Enteropathogenic E.coli, enterotoxigenic E.coli, enterohemorrhagic E.coli and enteroin-vasive E.coli were identified by analyzing four specific virulence genes. Results] A total of 32 bacterial strains were tested, giving no false-positive or false negative results. The detection limits were as low as: ETEC 27 CFU/mL, EPEC 33 CFU/mL, EHEC 25 CFU/mL and EIEC 42 CFU/mL. Conclusion] The data suggest that PCR-DHPLC will be useful for diarrheagenic Escherichia coli detection and identification.
%K PCR
%K DHPLC<br>致泻性大肠杆菌
%K 多聚酶链式反应
%K 变性高效液相色谱
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=A0C682BCB9B66E745CCEAAD38959C7EA&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=708DD6B15D2464E8&sid=28652329520A4AA7&eid=F41021172F25CDE0&journal_id=0001-6209&journal_name=微生物学报&referenced_num=8&reference_num=13