%0 Journal Article
%T Construction and toxicity the recombinant SpltMNPV expressing the scorpion toxin gene<br>表达蝎毒素的重组斜纹夜蛾核型多角体病毒(SpltMNPV)的构建及毒力
%A Xinxin Tang
%A Xinglu Sun
%A Guanqin Pu
%A Wenbing Wang
%A Chuanxi Zhang
%A Qilian Qin
%A Jiang Zhu
%A <br>汤欣欣
%A 孙兴鲁
%A 浦冠勤
%A 王文兵
%A 张传溪
%A 秦启联
%A 朱江
%J 微生物学报
%D 2011
%I 
%X Objective] To develop a high toxic recombinant Spodoptera litura multicapsid nucleopolyhedroviruse(SpltMNPV) insecticide.Methods] We constructed a recombinant transfer vector that was characterized by disrupting of ecdysterioid UDP-glucosyltransferase(egt) gene and expressing the mature peptide of the Chinese scorpion,B.martensi Karsch(BmK ITa1) gene at the control of ie-1 promoter.The transfer vector and the SpltMNPV II DNA co-transfected the SpLi cells.Recombinant viruses were purified by the end point ...
%K Spodoptera litura multicapsid nucleopolyhedroviruse(SpltMNPV)
%K B
%K martensi Karsch(BmK ITa1)
%K ecdysterioid UDP-glucosyltransferase(egt)
%K recombinant baculovirus<br>关键词:
%K 斜纹夜蛾核型多角体病毒(Spodoptera
%K litura
%K multicapsid
%K nucleopolyhedroviruse，SpltMNPV)，东亚钳蝎神经毒素基因(B．martensi
%K Karsch，BmK
%K ITa1)
%K ，蜕皮激素UDP-葡萄糖基转移酶基因(ecdysterioid
%K UDPglucosyl
%K transferase
%K gene，egt)，重组杆状病毒
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D1920AB56F28E37CBFB6D6AA3A975E85&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=708DD6B15D2464E8&sid=BD9BFF5428C0CED6&eid=AEA8CA70A6C58E24&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=24