%0 Journal Article
%T Cloning beta-tubulin from Fusarium fujikuroi to analyze its relationship with carbendazim resistance<br>水稻恶苗病菌(Fusarium fujikuroi)β-微管蛋白基因克隆及与多菌灵抗药性关系
%A Ma Xiaowei
%A Xing Chunjie
%A Yu Jinfeng
%A Wang Yong
%A Chen Zihao
%A Wang Jianxin
%A Zhou Mingguo
%A Chen Changjun
%A <br>Ma X
%A Xing C
%A Yu J
%A Wang Y
%A Chen Z
%A Wang J
%A Zhou M
%A Chen C
%J 微生物学报
%D 2012
%I 
%X Objective] We studied the resistance of Fusarium fujikuroi against carbendazim,to confirm whether the resistance was related to the β-tubulin gene.Methods] The isolated strains were identified based on TEF-1α gene sequence and morphological characteristics.The whole nucleotide sequence of β-tubulin gene was analyzed from different sensitive strains by the primers designed according to the nucleotide sequence of β-tubulin gene from the sequenced strain 7600 of F.verticillioides.Real-time quantitative RT-PCR(qRT-PCR) was applied to analyze the expression pattern of β-tubulin gene from different sensitive strains treated with or without carbendazim.Results] The full-length nucleotide sequence of β-tubulin gene(Accession Number: JQ026022) was cloned from different sensitive strains of F.fujikoroi,which spanned 1671 bp with 4 introns,encoding 447 amino acids.This gene showed 100% nucleotide sequence homology between sensitive and resistant strains.The expression of the β-tubulin gene in 2 sensitive strains was significantly higher than 3 resistant strains when these strains were cultured on carbendazim-free media(p=0.05).Carbendazim at the concentration of their EC50 caused the expression of the gene from the five strains increasing significantly(p=0.05) in comparison with that of strains treated without carbendazim.However,there was no significant difference between strains when treated with carbendazim.Conclusion] The resistance of F.fujikuroi against carbendazim was irrelative to β-tubulin gene.
%K Fusarium fujikuroi
%K β-tubulin gene
%K expression pattern
%K carbendazim-resistance<br>水稻恶苗病菌
%K β-微管蛋白基因
%K 表达特性
%K 多菌灵抗药性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=4D1F54CAF2A20CB3B7C9A5690A8939FF&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=94C357A881DFC066&sid=93ADA2AA3F969E58&eid=D5970ECA7D10A7B1&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0