%0 Journal Article
%T Agrobacterium tumefaciens mediated transformation of Aspergillus niger conidiospores and sequence analysis of TDNA insertion site in conidiation-defective mutant<br>农杆菌介导转化黑曲霉分生孢子及产孢缺陷突变子T-DNA插入位点分析
%A Wen Huang
%A Hongjiang Yang
%A Huibin Qin
%A <br>黄文
%A 杨洪江
%A 秦慧彬
%J 微生物学报
%D 2011
%I 
%X Abstract: Objective] Development of a method of transforming Aspergillus niger conidiospores with Agrobacterium tumefaciens T-DNA system and using it as a tool for genome annotation via construction a T-DNA insertion mutant library of A. niger. Methods] A. tumefaciens EHA105 carrying the binary vector pCAMBIA1301 was used to transform A. niger conidiospores under induction conditions. Hygromycin resistant transformants were screened on the selected medium. Sequence analysis was performed usimg inverse PCR method in stable mutants. Results] In total, 193 hygromycin resistant stable transformants were obtained and the transformation rate was about 5.6?102 transformants /108 conidiospores. Obvious morphological changes were observed in some mutants and sequence analysis of T-DNA inserted site was performed in one conidiation-defective mutant. The result showed that the interrupted gene belonged to major facilitator superfamily (MFS). Conclusion] Our results indicated that the transformation system of A. niger conidiospores set up in this study, combing with sequence analysis of T-DNA insertion site, might be an effective way for genome annotation in A. niger.
%K Keywords: Aspergillus niger conidiospores
%K Agrobacterium tumefaciens mediated transformation
%K T-DNA
%K Inverse PCR<br>关键词：黑曲霉分生孢子
%K 农杆菌介导转化
%K T-DNA
%K 反向PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6D9E70D184F43EEF961E72006C707F51&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=0B39A22176CE99FB&sid=B7BFA4B351E4C682&eid=7979125BBE749348&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=18