%0 Journal Article
%T Cloning and expression of luxS and pfs and in vitro biosynthesis autoinducer 2 of avian pathogenic Escherichia coli from Anhui Province<br>禽致病性大肠杆菌安徽分离株luxS和pfs基因的克隆、表达与细胞外合成AI-2 活性检测
%A Xiangan Han
%A Hao Bai
%A Lei Liu
%A Wenjing Chen
%A Chan Ding
%A Qinghai Hu
%A Kezong Qi
%A Shengqing Yu
%A <br>韩先干
%A 白灏
%A 刘蕾
%A 陈文静
%A 丁铲
%A 胡青海
%A 祁克宗
%A 于圣青
%J 微生物学报
%D 2012
%I 
%X Objective] The LuxS/AI-2 quorum sensing(QS) system shared by Gram-positive and Gram-negative bacteria involves the production of autoinducer-2(AI-2).In this study,the method of biosynthesis of AI-2 was established using recombinant LuxS and Pfs of avian pathogenic Escherichia coli(APEC),which will be benefit for future study of the role of AI-2 in APEC.Methods] We investigated AI-2 production in APEC by vibrio harveyi BB170(BB170).Furthermore,APEC LuxS and Pfs were expressed,purified and used to investigate the production of AI-2 in vitro.LuxS and Pfs were incubated with S-ribosylhomocysteine(SAH),the reaction was detected for the production of luminescence of BB170.Results] APEC can produce AI-2 by BB170 bioassay.Purified LuxS and Pfs enzymes incubated with SAH and produced 300μmol/L AI-2 in the reaction products.Conclusion] The results demonstrated that recombinant Pfs and LuxS synthesize AI-2 in vitro from SAH.These findings will be of benefit to future studies of the role of AI-2 in APEC.
%K Avian Pathogenic Escherichia coli
%K quorum sensing
%K autoindure-2
%K LuxS<br>禽致病性大肠杆菌
%K 密度感应系统
%K 信号分子AI-2
%K LuxS
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=2350B5576F89D072A9EFCBC54F8649AB&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=9CF7A0430CBB2DFD&sid=9905045FEE51B18F&eid=B9196C90508452FE&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0