%0 Journal Article
%T Lactobacillus acidophilus NCFM affects host adhesion-related gene expression after adhering to host<br>嗜酸乳杆菌NCFM粘附宿主后对其粘附相关基因表达的影响
%A Linlin Han
%A Chaoxin Man
%A Xuena Lv
%A Mingna Wang
%A Guanghui Zhang
%A Ying Liu
%A Yujun Jiang
%A <br>韩琳琳
%A 满朝新
%A 吕学娜
%A 王明娜
%A 张光辉
%A 刘颖
%A 姜毓君
%J 微生物学报
%D 2011
%I 
%X Abstract: Objective] Adhering to the intestinal epithelial cells is one of the beneficial functions exerting by probiotics. We tested and verified the expression of adhesion-related genes in vivo and in vitro to understand the influence from Lactobacillus acidophilus NCFM on host cells. Method] We selected the adhesion-related genes through GO (gene ontology) category from the Human Genome U133 Plus 2.0 Array analysis. These genes were verified by in vitro Caco-2 cells culture model and in vivo mouse model using Real-time PCR method. Results] After L. acidophilus NCFM adhering to Caco-2, we found that 12 adhesion-related genes were up-regulated. The up-regulation was confirmed in vivo and in vitro by Real-time PCR assay. Among them, the up-regulated expression of chemokine (C-C motif) ligand 2 (CCL2) gene was the most distinguished one. Conclusion] Our research showed that L. acidophilus NCFM, adhering to Caco-2 cells, could cause the differential expression of the host adhesion-related genes. Results of this study seemed to provide some useful data for further revealing its effect on intestinal epithelial cells.
%K Keywords: Lactobacillus acidophilus NCFM
%K Caco-2 cells
%K adhesion
%K gene expression<br>关键词：嗜酸乳杆菌NCFM，Caco-2细胞，粘附，基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6DFF9D25C5FF93979279BA358E17F595&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=94C357A881DFC066&sid=5568599C60D4BE87&eid=2C20277AC27E4821&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=14