%0 Journal Article
%T Construction and characterization of a brucella suis S2 strain with a chloramphenicol resistance marker<br>带有氯霉素抗性基因标记的重组布氏杆菌S2的构建及其生物学特性
%A MAO Kai-rong
%A DING Jia-bo
%A CHENG Jun-sheng
%A JIANG Yu-wen
%A YAO Wen-sheng
%A <br>毛开荣
%A 丁家波
%A 程君生
%A 蒋玉文
%A 姚文生
%J 微生物学报
%D 2007
%I 
%X Vaccination has not been used widely because of the interference in the discrimination between infected and vaccinated animals in immune-screening procedures. In the present study,chloramphenicol resistance gene(Cm~r)was cloned into the genomic DNA of brucella suis S2 strain by homologous recombination with knocking out the WbkC gene,and obtained the recombinant rS2-WbkC. Further study confirmed that rS2-WbkC was conversed into rough-phenotype form smooth-phenotype. The recombinant keeps the ability to chloramphenicol resistance after 25 passages in tryptic soy agar (TSA). Mice tests showed rS2-WbkC offered similar protection to S2 strain,but more safe than S2. Serum collected form rS2-WbkC immunized mice could be easily distinguished from antiserum produced by smooth-phenotype brucella abortus. In view of these result,rS2-WbkC is a promising candidate for vaccine strain.
%K brucell suis
%K chloramphenicol resistance gene
%K WbkC gene
%K vaccine strain<br>布氏杆菌
%K 氯霉素抗性基因
%K WbkC基因
%K 标记疫苗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=ED2CC2FD33A6F13BB980942FF9EB7E91&yid=A732AF04DDA03BB3&vid=F4B561950EE1D31A&iid=B31275AF3241DB2D&sid=32491EEEE0A8C927&eid=90C2E72D2E105FF5&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=8