%0 Journal Article
%T Analysis of T-DNA integration pattern in Botrytis cinerea genome<br>灰葡萄孢菌（Botrytis cinerea）基因组中T-DNA整合模式分析
%A Jia Liu
%A Jianyun Zhang
%A Tingheng Zhu
%A Kun Wang
%A Zhifeng Cui
%A <br>刘佳
%A 张剑云
%A 朱廷恒
%A 汪琨
%A 崔志峰
%J 微生物学报
%D 2011
%I 
%X Abstract: Objective] To analyze the T-DNA integration pattern in the genome of grey mold Botrytis cinerea. Methods] T-DNA (Transfer DNA) inserted mutant library of Botrytis cinerea was created by Agrobactirium tumfacience mediated transformation. By using TAIL-PCR (Thermal asymmetric interlaced polymerase chain reaction), we amplified and cloned the chromosomal regions flanking T-DNA insertions. The obtained T-DNA flanking sequences were subjected to alignment with standard T-DNA border sequence for identification and analysis of integration. Results] Up to 69% T-DNA inserted at noncoding regions and 30% inserted at exons. Recombination including deletion or addition of bases in T-DNA region was observed. The right borders of the T-DNA were frequently truncated, and by contrast the left borders were less prone to degradation and appeared to have been inserted in a relatively integrated manner. Extra sequence additions also occurred in T-DNA integration sites. Conclusion] Analysis of T-DNA integration pattern in B. cinerea genome will stimulate the functional genomics study of this fungus.
%K 关键词：灰葡萄孢，T-DNA，TAIL-PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=C5E1A5C2200FB3A583C5809F04F412D2&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=0B39A22176CE99FB&sid=38685BC770C663F2&eid=334E2BB8B9A55ABB&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=13