%0 Journal Article %T Diversity of C16 H33 Cl-degrading bacteria in surface seawater of the Arctic Ocean
北极表层海水中氯代十六烷降解菌的多样性 %A Jianning Wang %A Chunming Dong %A Qiliang Lai %A Longshan Lin %A Zongze Shao %A
王建宁 %A 董纯明 %A 赖其良 %A 林龙山 %A 邵宗泽 %J 微生物学报 %D 2012 %I %X Objective]To detect the diversity of the degradation bacteria of halogenated-alkane form the surface seawater of the Arctic Ocean.Method] Twelve surface-water samples from the Arctic Ocean were collected and enriched using C16H33Cl as the sole carbon and energy source.Bacteria from the enriched cultures were isolated on marine agar,and followed by 16S rRNA gene identification and phylogenetic analysis.Further,their degradation ability was tested with C16H33Cl.The bacterial community structures were further examined by denaturing gradient gel electrophoresis(DGGE).Result] In total 112 isolates were obtained from the 12 samples,of which 19 isolates degraded C16H33Cl.Bacteria of Alcanivorax and Rhodococcus exerted good emulsification and degradation,whereas bacteria of Marinobacter also had the degradation capacity,but less.DGGE analysis revealed that Alcanivorax,Parvibaculum and Thioclavawere dominated in the enriched consortia.Conclusion] The C16H33Cl degradation bacteria in the Arctic marine environment mainly belonged to α-proteobacteria,γ-proteobacteria,actinobacteria and bacteroidetes.This is the first report on the diversity of degradation bacteria of halogenated alkane in the Arctic Ocean.Our result contributed to the knowledge about the arctic environment and the biodiversity of degrading bacteria. %K the Arctic %K surface seawater %K C16H33Cl %K biodegradation %K diversity
北极 %K 表层海水 %K 氯代十六烷 %K 生物降解 %K 多样性 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=1D086AF23E396C1E028B80B6FC2E4C65&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=5D311CA918CA9A03&sid=BCE65990EF9348F5&eid=E947FD4445DA7BA0&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0