%0 Journal Article
%T Archaeal diversity in sediment of Dunbasitawu salt lake, Xinjiang, China, estimated by 16S rRNA gene sequence analyses<br>新疆顿巴斯他乌盐湖沉积物免培养古菌多样性
%A Li Zhu
%A Hongmei Yang
%A Yun Wang
%A Xianyan Liao
%A Lijuan Deng
%A Shan Li
%A Kai Lou
%A Erkin Rahman
%A <br>朱莉
%A 杨红梅
%A 王芸
%A 廖先燕
%A 邓丽娟
%A 李珊
%A 娄恺
%A 艾尔肯·热合曼
%J 微生物学报
%D 2012
%I 
%X Objective]We surveyed the composition and diversity of uncultured archaea in Xinjiang Dunbasitawu salt Lake sediment.Methods] Environmental total DNA was directly extracted from the sediment.We constructed clone library of 16S rRNA gene amplified with archaea-specific primers.A total of 59 positive clones were randomly selected from the library and identified by restriction length polymorphism(RFLP) with enzyme Hae Ⅲ.Clones with the unique RFLP pattern were sequenced,and then by phylogenetic analysis.Results] The clone coverage C value was 89%,and Shannon-Wiener index was 2.69.In total,21 Operational Taxonomic Units(OTUs) were obtained and affiliated with Euryarchaeota(92%) and Crenarchaeota(8%).The most of clones were affiliated to Halobacterium(24%),Haloarcula(18%),Natronorubrum(14%),and Halorubrum(8%),which belonged to family Halobacteriaceae(88%) with high similarity to that from thalassohaline environment.In addition,11% of clones had less than 97% similarity with archaea sequences deposited in GenBank database.Conclusion] Compared with other similar Hypersaline environments,archaea diversity in Dunbasitawu salt lake was a little lower.The proportion of archara was different,but the composition is consistent.It was implied that some potential new species or lineages maybe exist in Dunbasitawu salt lake.
%K Hypersaline environment
%K salt lake
%K thalassohaline
%K archaea diversity
%K 16S rRNA homogeny<br>高盐环境
%K 盐湖
%K 海盐环境(thalassohaline)
%K 古菌多样性
%K 16S
%K rDNA同源性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6872F7A14063ADE5C73AFA752941C29B&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=B31275AF3241DB2D&sid=CA122BD5B2FF2137&eid=FA519F4FF622280A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0