%0 Journal Article
%T Cloning, sequencing and identification of replication origin of Rhodococcus linear plasmid pNSL<br>红球菌线型质粒pNSL1的克隆、测序和复制区的鉴定
%A Yingmin Zhu
%A Mingxuan Xu
%A Meijuan Shen
%A Zhenhua Chen
%A Zhongjun Qi?n
%A <br>朱颖旻
%A 许铭翾
%A 沈美娟
%A 陈振华
%A 覃重军
%J 微生物学报
%D 2010
%I 
%X Abstract: Two linear plasmids, pNSL1 and pNSL1, were detected from Rhodocuccus sp. NS1. Objective] Cloning, sequencing and identification of replication origin of the Rhodococcus linear plasmid pNSL1. Methods] Large amount of linear plasmid DNA was recovered from pulsed-field gels for shotgun-cloning and sequencing, and identification of its replication locus. Results] The complete nucleotide sequence of pNSL1 consisted of 117252 bp, including the conserved 1282-bp telomere sequences among Rhodococcus linear plasmids. pNSL1 encoded 103 open reading frames, including functions of replication, maintenance and transfer etc. A locus, pNSL1.038 and upstream 767-bp non-coding sequence, was identified for autonomous replication by cloning in an E. coli vector and introduced by electroporation into Nocardia coralline 4.1040. Conclusion] Cloning and sequencing of Rhodococcus linear plasmid pNSL1, and identification of its replication origin.
%K Keywords: Rhodococcus
%K linear plasmid
%K telomere
%K replication origin<br>关键词：红球菌
%K 线型质粒
%K 端粒
%K 复制
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=56719E6FEED3EE7472EC24F00EE95FE3&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=5D311CA918CA9A03&sid=4759DE41370E2E7A&eid=17FE7A1C78626A81&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0