%0 Journal Article
%T Purification and Characterization of Thermostable Amylases from Two Bacterial Species<br>嗜热菌来源的生淀粉酶分离纯化及其酶学性质
%A Yongcun Dong
%A Yang Liu
%A Yuanyuan Chen
%A Dandan Niu
%A Liang Zhang
%A Guiyang Shi
%A Zhengxiang Wang
%A <br>董永存
%A 刘洋
%A 陈源源
%A 牛丹丹
%A 张梁
%A 石贵阳
%A 王正祥
%J 微生物学报
%D 2008
%I 
%X Two thermophilic bacterial isolates, strain 173 and strain 174, with raw starch-digesting activities were selected from thermophilic bacteria growing in hot spring of Tengchong County, Yunnan Province, China. By amplification, sequencing and alignment analysis of 16S ribosomal DNAs, they were identified as members of genus Geobacillus. In shaker flask culture Geobacillus sp. 173 produced 14.5 U/mL amylase and for Geobacillus sp. 174 with 12.9 U/mL . Both amylases were purified by starch absorption-desorption and chromatograph. The amylases from strain 173 and strain 174 purified to about 50 and 29 folds were respectively achieved with an overall yield of 34% and 41%. The maximum gelati-nized-starch-digesting activity of the purified amylases were at 70°C and pH 5.0~5.5. The high raw-starch-digesting ac-tivity of these enzymes were observed at 50°C~60°C ( from strain 173) and 40°C ~60°C (from strain 174). Both enzymes were not sensitive to ions including mental ions (Na+, K+, Mg2+, Ca2+, Mn2+, Zn2+) and others (EDTA, Citrate), but were slightly inhibited by ions such as Co2+, Cu2+ for amylase from strain 173 and Cu2+ for amylase from strain 174. Both en-zyme had specificity of starch substrates.
%K thermophilic bacilli
%K raw-starch-digesting amylase
%K purification and characterization<br>嗜热菌
%K 生淀粉酶
%K 分离纯化
%K 嗜热菌
%K 来源
%K 淀粉酶
%K 分离纯化
%K 酶学性质
%K Species
%K Bacterial
%K Amylases
%K Characterization
%K 比值
%K 糊化淀粉
%K 降解
%K 玉米淀粉
%K 木薯淀粉
%K 小麦淀粉
%K 红薯淀粉
%K 底物专一性
%K 抑制作用
%K 酶活力
%K 敏感性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=0573F15062044105B12E0AA31745FEE0&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=0B39A22176CE99FB&sid=954CE65414DD94CA&eid=A58CF3BAE79427D0&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=22