%0 Journal Article
%T Analysis of the chromosome ploidy of Candida glycerinogenes<br>产甘油假丝酵母(Candida glycerinogenes)染色体倍性分析
%A Baoping Song
%A Bin Zhuge
%A Huiying Fang
%A Jian Zhuge
%A <br>宋保平
%A 诸葛斌
%A 方慧英
%A 诸葛健
%J 微生物学报
%D 2011
%I 
%X Abstract: Objective] To determine the chromosome DNA ploidy of Candida glycerinogenes. Methods] We screened the haploid cell of Saccharomyces cerevisiae and the diploid of Candida tropicalis as the reference cell to identify the ploidy of Candida glycerinogenes. The concentrations of chromosome DNA extracted from both haploid and diploid cells were determined by the diphenylamine reaction method. Meanwhile, the cell number of Candida tropicalis, Candida glycerinogenes, Saccharomyces cerevisiae and its haploid were accounted by hemocytometer measurement method, respectively. Because in the same UV irradiation conditions, the haploid cells was more easy died than diploid cells, so we used the UV irradiation test to further verification. Results] The results of the content of chromosome DNA in a single cell showed that Candida glycerinogenes was a diploid cell. Moreover, it was also further confirmed because the haploid cell of Saccharomyces cerevisiae was more sensitive than Candida glycerinogenes under UV treatment. Conclusion] By this study, we confirmed that Candida glycerinogenes was a diploid cell.
%K Keywords: sporulation
%K haploid
%K Candida glycerinogenes
%K ploidy<br>关键词：产孢条件
%K 单倍体
%K 产甘油假丝酵母
%K 染色体倍性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=F8B924B609564F3D0594972FFFEC47B0&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=38B194292C032A66&sid=377D325742940769&eid=717CC18E05F2AFA0&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0