%0 Journal Article
%T Isolation and identification of a novel Candida sp. H2 producing D-arabitol and optimization of D-arabitol production<br>产D-阿拉伯醇菌株的筛选、鉴定及其产D-阿拉伯醇条件的优化
%A Weibin Song
%A Yanqing Lin
%A Haiyan Hu
%A Zhipeng Xie
%A Jianguo Zhang
%A <br>宋卫斌
%A 林燕青
%A 胡海燕
%A 谢志鹏
%A 张建国
%J 微生物学报
%D 2011
%I 
%X Abstract: Objective] To isolate a new osmophilic yeast for producing D-arabitol and research its optimal fermentation conditions for highest yield of D-arabitol from glucose. Methods] The isolated strain was characterized by electron microscopy, Biolog (GN) test, G+C content measurement and 26S rDNA D1/D2 domain sequences analysis. The purified fermentation product was identified by IR, 1H-NMR, 13C-NMR, MS and optical rotation analysis. Then the fermentation conditions for D-arabitol production were optimized. Results] A new osmophilic yeast was isolated and identified as Candida sp. H2. Through the single factor experiment, the optimum conditions of 250 g/L glucose, 10 g/L yeast extract, initial pH 6.0, 35 °C of culture temperature, 200 rpm of agitation, 200 mL medium in a 1000-mL flask of broth content, 1% (v/v) of inoculum size, 96 h of fermentation time were achieved. Based on the conditions above, weight yield of 35% (86.55 g D-arabitol from 250 g glucose) was obtained and 10% higher than the conditions not optimized. Conclusions] Candida sp. H2 was a novel strain for producing D-arabitol and valuable for further study.
%K Key words: Polyalcohol
%K D-arabitol
%K Xylitol
%K Osmophilic yeast
%K Candida<br>关键词：多羟基醇，D-阿拉伯醇，木糖醇，耐高渗酵母，假丝酵母
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=4037E2BDE3E3E0F51BDA4D7B46A6A7E7&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=38B194292C032A66&sid=2E01F39B6CBD53DE&eid=5A6705FDACED0BF9&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=21