%0 Journal Article
%T Construction and characterization of Listeria monocytogenes ΔprfA mutant strains<br>单核细胞增生性李斯特菌prfA基因缺失株的构建及其生物学特性
%A Chunguang Bai
%A Yuelan Yin
%A Yanyan Jia
%A Hong Fu
%A Yunfei Gao
%A Xin'an Jiao
%A <br>白春光
%A 殷月兰
%A 贾艳艳
%A 付红
%A 高云飞
%A 焦新安
%J 微生物学报
%D 2011
%I 
%X Objective] Listeria monocytogenes(Lm) is an important pathogen that can cause serious listeriosis in humans and animals.The pathogenicity of Lm has a close relationship with the PrfA protein regulating the expression of virulence genes.Therefore,we studied the regulation functions of PrfA and its role on Lm's virulence.Methods] The prfA genes of LM4,serotype 1/2a,and F4636,serotype 4b,were deleted by homologous recombination technology,and the biological characteristics of the mutants were further studied...
%K Listeria monocytogenes
%K mutant
%K prfA
%K homologous recombination
%K biological characteristics<br>关键词:
%K 单核细胞增生性李斯特菌，突变株，prfA
%K 基因，同源重组，生物学特性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=D1920AB56F28E37C0783864A219C6998&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=708DD6B15D2464E8&sid=43797DD45B2BF7AC&eid=0E6C9B38667ABC5C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=15