%0 Journal Article
%T Characterization of root nodule and rhizobium of a leguminosae ephemeral plant- Trigonella arcuata C.A.Mey in Xinjiang<br>新疆豆科短命植物弯果胡卢巴根瘤与根瘤菌的特性
%A Tianyu You
%A Zhi Tan
%A Lili Gu
%A Xiuming Li
%A Shixiang Yao
%A Haiyan Lan
%A Fuchun Zhang
%A <br>油天钰
%A 谭植
%A 谷丽丽
%A 李秀明
%A 姚世响
%A 兰海燕
%A 张富春
%J 微生物学报
%D 2008
%I 
%X OBJECTIVE: We studied root nodule proliferation, nodule microstructure, genetic cluster and stress resistance of the rhizobium of Trigonella arcuata. METHODS: We characterized root nodule and rhizobium with various soil matrixes cultivation, paraffin section, resin semi-ultrathin section techniques, and 16S rRNA gene cluster analysis. RESULTS: (1) Plants grew in mixed soil (nutritious garden soil: poplar zone soil: desert sands = l:1:1), had the most nodule proliferation and bore the most pods. The shapes of nodule were palm- or ginger-like; (2) Microstructure of the nodule revealed five different parts differentiated within the nodule: epidermis (E), cortex (C), vascular bundle (VB), infected cells (IC) and uninfected cells (UIC); (3) Genetic cluster analysis of the full length 16S rRNA gene sequence (1377 bp) indicated that the rhizobium isolated shared the highest identities with Sinorhizobium meliloti; (4) The rhizobium could grow between 4 and 60 degrees C (20 min), pH 6.0-12.0 and 0-2% NaCl. For the antibiotic sensitivity, the rhizobium could not grow normally in medium with 25 microg/mL Kanamycin, Streptomycin or Cephalothin, except for 100 microg/mL Ampicillin. CONCLUSION: Good conditions of soil matrixes were important for nodulation of T. arcuata; A large quantity of cells in fascicular nodules were infected by rhizobia; 16S rRNA gene sequence of T. arcuata shared the highest identities with that of Sinorhizobium meliloti, and this strain was able to tolerate relatively higher temperature and alkalin.
%K leguminosae ephemeral plant
%K Trigonella arcuata C
%K A
%K Mey
%K root nodule
%K rhizobium
%K microstructure
%K 16S rRNA gene
%K stress resistance<br>豆科短命植物
%K 弯果胡卢巴
%K 根瘤
%K 根瘤菌
%K 显微结构
%K 16S
%K rRNA基因
%K 抗逆性
%K 新疆
%K 豆科
%K 短命植物
%K 根瘤菌
%K 特性
%K leguminosae
%K root
%K Characterization
%K 菌株
%K 强碱
%K 耐高温
%K 细胞
%K 侵染
%K 瘤内
%K 通气条件
%K 土壤
%K 氨苄青霉素
%K 完全
%K 抗生素
%K 头孢霉素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=BC8EC7F06220BB13B59252A99BFCBCE0&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=DF92D298D3FF1E6E&sid=3D9E2C3DB640307A&eid=3019419AF3C0F2FB&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=24