%0 Journal Article
%T Toxicity screening and identification of bacteria isolated from snails Nassarius semiplicatus and their habitat<br>半褶织纹螺(Nassarius semiplicatus)及其生活环境中分离培养的细菌毒性测试与种类分析
%A Xiaojie Wang
%A Rencheng Yu
%A Xuan Luo
%A Mingjiang Zhou
%A Jinjin Shen
%A Zhenguo Gu
%A <br>王晓杰
%A 于仁成
%A 罗璇
%A 周名江
%A 沈进进
%A 顾振国
%J 微生物学报
%D 2008
%I 
%X Objective] Tetrodotoxin and its analogues (TTXs) were responsible for the poisoning incidents associated with snail Nassarius spp. We studied bacteria isolated from toxic snails as well as their habitat to probe into the relationship between bacteria and toxicity of nassariid gastropod. Methods] Two snail samples were collected from Sheyang, Jiangsu Province on June 13 and 19, 2006, and the toxicity of the snail samples was tested with mouse bioassay method. Bacteria were isolated from the snail samples and from their habitat. A part of isolated bacteria were then cultured in the lab, and TTX in bacteria were screened with an ELISA method. Results] The snails collected were identified as Nassarius semiplicatus, and the toxicity of the 2 samples were 247 mouse unit (MU) and 270 MU / 100g tissue (wet weight), respectively. TTX was detected in 9 strains among the 14 strains of bacteria isolated from the snail samples and their habitat. TTX content in the toxic strains was very low, which ranged from 15 ng/g to 96 ng/g. Partial of the 16S ribosomal DNA (rDNA) of the toxic strains were then sequenced after PCR amplification, and the toxic strains of bacteria were tentatively identified based on the alignment of these sequences with published data. Toxic strains were closely affiliated with Vibrio, Shewanella, Planococcus, Marinomonas, Photobacterium. Conclusion] These findings suggested that TTX-producing bacteria may play an important role in TTX production or accumulation in nassariid gastropod.
%K Nassarius spp
%K tetrodotoxin
%K bacteria
%K ELISA
%K 16S rDNA<br>织纹螺
%K 河豚毒素
%K 细菌
%K ELISA
%K 16S
%K rDNA
%K 半褶织纹螺
%K 生活环境
%K 分离培养
%K 细菌
%K 毒性测试
%K 种类
%K 分析
%K habitat
%K isolated
%K bacteria
%K identification
%K screening
%K 存在
%K 发现
%K 亲缘关系
%K 相似性
%K 菌属
%K Photobacterium
%K 发光杆菌
%K 单胞菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6CAD01B31B5F580542EDD6D9F91F6D08&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=DF92D298D3FF1E6E&sid=58AAF01A97187A3A&eid=DE4E739E935BD9A7&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=27