%0 Journal Article
%T Expression of PTX3 gene in Caco-2 cells treated with Lactobacillus acidophilus NCFM<br>嗜酸乳杆菌NCFM对Caco-2细胞中PTX3基因表达的影响
%A Xuena Lv
%A Chaoxin Man
%A Linlin Han
%A Mingna Wang
%A Guanghui Zhang
%A Ying Liu
%A Shiqin Yang
%A Yuqing Xue
%A Yujun Jiang
%A <br>吕学娜
%A 满朝新
%A 韩琳琳
%A 王明娜
%A 张光辉
%A 刘颖
%A 杨士芹
%A 薛玉清
%A 姜毓君
%J 微生物学报
%D 2011
%I 
%X Objective]To study the expression of immunity and inflammatory mediator factor PTX3 in intestinal epithelial cells treated with Lactobacillus acidophilus NCFM and further to reveal the regulatory mechanism.Methods]Caco-2 cells were cocultured with Lactobacillus acidophilus NCFM for 0,2,4,8,12 h and 0,0.5,1,2,4 h respectively,then the total RNA and protein were extracted.The expression of PTX3 gene was analyzed by Real Time RT-PCR.The phosphorylation levels of NF-κB was analyzed by Western Blot.Caco-2 cells were pretreated with PDTC for 30 min before cocultured with Lactobacillus acidophilus NCFM for 2 h,then the total RNA was extracted and the expression of PTX3 gene was analyzed by Real Time RT-PCR.Results]Lactobacillus acidophilus NCFM could induce the expression of PTX3 in Caco-2 cells.The PTX3 expression peaked at 4 h after coculture.Then its expression gradually waned out.Lactobacillus acidophilus NCFM could rapidly activate the phosphorylation of NF-κB,and the expression of PTX3 was decreased notably after pretreated with PDTC for 30 min.Conclusion] Lactobacillus acidophilus NCFM could transiently regulate the immunity and inflammatory mediator factor PTX3 expression through rapidly activating NF-κB signaling pathway in Caco-2 cells.
%K Lactobacillus acidophilus NCFM
%K Caco-2 cells
%K PTX3 gene
%K NF-κB signaling pathway<br>嗜酸乳杆菌NCFM
%K Caco-2细胞
%K PTX3基因
%K NF-κB途径
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=91FFA20C56B114156F29AE2B9447123E&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=E158A972A605785F&sid=D8414BC1307BF1A3&eid=A3F93694B058F76C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=19