%0 Journal Article
%T Detection of Escherichia coli O157:H7 with piezoelectric immunosensor based on enhancement with immuno-nanoparticles<br>基于免疫纳米颗粒强化的压电免疫传感器检测大肠杆菌O157︰H7
%A Zhiqiang Shen
%A Jingfeng Wang
%A Zhigang Qiu
%A Min Jin
%A Xinwei Wang
%A Zhaoli Chen
%A Junwen Li
%A Fuhuan Cao
%A <br>谌志强
%A 王景峰
%A 邱志刚
%A 金敏
%A 王新为
%A 陈照立
%A 李君文
%A 晁福寰
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] We developed a sensitive method to detect Escherichia coli O157:H7 based on enhancement with immuno-nanoparticles. Method] About 10 nm ultrafine immunomagnetic particles were prepared by a co-precipitation method, about 20 nm immuno-colloid-gold was prepared by sodium citrate reduction. The immunosensor was fabricated by using Protein A from Staphylococcus aureus (SPA) for the antibody immobilization. Two immuno-nanoparticles by two antibodies were used to amplify frequency changing. Result] The most suitable immobilization concentration and time for SPA was 1.2 mg/ml and 40 min, and dose for the antibody was 1.0 mg/ml and 60 min. With the signal amplification of the two immuno-nanoparticles, the detection limit of E. coli O157 : H7 was increased from 104 colony-forming units (cfu) /mL to 101 cfu/mL. Conclusion] The prepared immuno-nanoparticles might be used to significantly amplify the frequency signal of the piezoelectric immunosensor and improve the detect sensitivity.
%K Keywords: Escherichia coli O157:H7
%K piezoelectric immunosensor
%K immuno-nanoparticles
%K enhancement
%K detection<br>关键词：大肠杆菌O157︰H7
%K 压电免疫传感器
%K 纳米免疫磁颗粒
%K 免疫胶体金
%K 增强
%K 检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=10655481402906B9AB9CA1CE706452E7&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=B31275AF3241DB2D&sid=45D68DF69BA881EC&eid=95780E43ADDDE2AA&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=26