%0 Journal Article
%T Study on Low-temperature Alkaline Lipase from Bohaisea-9145<br>Bohaisea-9145海洋低温碱性脂肪酶研究
%A Tie-Juan Shao
%A Mi Sun
%A Jia-Sheng Zheng
%A Yue-Jun Wang
%A Xiu-Bao Qiu
%A <br>邵铁娟
%A 孙谧
%A 郑家声
%A 王跃军
%A 邱秀宝
%J 微生物学报
%D 2004
%I 
%X Lipase-overproducing marine yeast Bohaisea-9145 isolated from the Bohai sea region was identified as psychrotrophlic Yarrowia lipolytica. The optimum composition of culture medium was ground soybean 4%, rice bran 4%, peanut power 4%, crude peanut oil 0.5%, MgSO4 0.05%, KH2PPO4 0.2%. The optimum temperature and pH were 26 +/- 1 degrees C pH5.0, the time of fermentation cycle was 23h. The lipase activity was improved to 258.67 U/mL through optimization, four-fold more than that of initial strain. The lipase displayed maximum activity at pH 8.5, 35 degrees C and was stable within the range of pH 4.0 - 9.0 at low temperature. It had high thermosensitivity, good compatibility with familiar metal ions and chemical reagents. It was antioxidant and had strong resistance to high salinity. As a novel marine low-temperature lipase, it has promising prospect in many fields, especially as an additive of detergent.
%K Marine yeast
%K Low temperature
%K Alkaline lipase<br>海洋酵母
%K 低温
%K 碱性脂肪酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=3626D0EF50338C88&yid=D0E58B75BFD8E51C&vid=1AE5323881A5ECDC&iid=B31275AF3241DB2D&sid=7B927C26AC9ED104&eid=8225A9F184D4F1CA&journal_id=0001-6209&journal_name=微生物学报&referenced_num=16&reference_num=23