%0 Journal Article
%T Influence of cross-protection on the survival of Lactobacillus casei ATCC 393<br>交互保护对干酪乳杆菌ATCC 393~(TM)存活的影响
%A Feng Xue
%A Juan Zhang
%A Guocheng Du
%A Jian Chen
%A <br>薛峰
%A 张娟
%A 堵国成
%A 陈坚
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] In this study, we investigated the cross-protection of Lactobacillus casei ATCC? 393TM under multi-stress conditions. Methods] Cells pre-adapted to mild conditions (heat, H2O2, acid or bile salts) were then treated at lethal temperature (>60 oC) or hydrogen peroxide stress (>5 mmol/L). Furthermore, the changes of survival rate, intracellular pH and membrane fatty acid under lethal conditions with or without acid adaption were compared. Results] The cross-protection in Lactobacillus casei ATCC 393TM were affected by different stress conditions cell encountered. Acid pre-adaption, especially hydrochloride treatment, would increase the resistance of cells to lethal heat and peroxide stresses significantly, with the survival rate of 305-fold and 173-fold, respectively. Further study suggested that the effect of acid pre-adaption might be related to the regulation on intracellular pH and the saturation of cell membrane. Conclusion] In this study, hydrochloride adaption was the best inducer for the cross-protection of Lactobacillus casei ATCC 393TM to maintain relatively stable physiological status of cells. The results above supplied a novel way to investigate the relationship between different protective mechanisms in L. casei under different kinds of stresses.
%K Key words: Lactobacillus casei
%K cross-protection
%K stress resistance
%K intracellular pH
%K membrane fatty acid<br>关键词：干酪乳杆菌
%K 交互保护
%K 胁迫抗性
%K 胞内pH
%K 膜脂肪酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6DDE4F5CF506BE0D0529D74D9E46E33C&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=E158A972A605785F&sid=A94616C4391AA5EF&eid=D8B37A0210DB6B9D&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0