%0 Journal Article
%T Exploiting bioactive Enediynes from marine microbe based on activity and gene screening<br>基于活性和基因从海洋微生物中筛选烯二炔类抗生素
%A Gang Pei
%A #
%A Huanqin Dai#
%A Biao Ren
%A Xiangyang Liu
%A Lixin Zhang
%A <br>裴刚
%A 代焕琴
%A 任彪
%A 刘向阳
%A 张立新
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] A high quality library of marine microbes and their associated natural products is critical for successful drug discovery. In this research a method to assess the quality of our marine microbial natural product library through screening for novel enediyne-like compounds from this library was set up. Methods] A high throughput screening assay based on the unique DNA-damage activity of enediyne-like compounds has been constructed and our marine microbial natural product library was screened. Because the polyketide synthase responsible for the biosynthesis of enediyne core is a conserved iterative type I polyketide synthase, a sequence-based screening method was built to get the strains that harbored enediyne biosynthesis gene clusters. Results] Through activity-based screening, a positive hit from our marine natural product library was acquired. 16S rRNA analysis has revealed that this strain-LS481 was 99% similar to Micromonospora chersina DSM 44151T that could produce enediyne compound-Dynemicins. The crude extract of LS481 showed similar characteristics with Dynemicin A. In addition, two strains, MS098 and LS2004, were acquired through sequence-based screening. The 16S rRNA of MS098 was 100% the same as Streptomyces griseus NBRC 13350, and LS2004 was most close to Streptomyces vinaceus NBRC 13425T and Streptomyces cirratus NRRL B-3250T. Conclusion] We can successfully isolate enediyne compounds through the activity-based screening and assess the potential of producing enediyne compounds through sequence-based screening.
%K Keywords: Enediyne
%K High throughput screening
%K Iterative type I polyketide synthase<br>关键词：烯二炔类抗生素
%K 高通量筛选
%K 重复I型聚酮合成酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=AE8EC4EE77C50E437CB7002B7A0EA569&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=E158A972A605785F&sid=7D1E6EEC2019967D&eid=F18BA6286A889C1C&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=0