%0 Journal Article
%T Diversity of neutrotolerant acidophilic streptomycetes isolated from acidic soils in Yunnan Province<br>云南酸性土壤中度嗜酸链霉菌的多样性
%A Chunguang Xu
%A Xiumei Liang
%A Ying Huang
%A Liming Wang
%A Qingfeng Cui
%A <br>徐春光
%A 梁秀梅
%A 黄英
%A 王黎明
%A 崔庆峰
%J 微生物学报
%D 2008
%I 
%X Objective] Acidophilic filamentous acitnomycetes are active in the turnover of organic matter in acid litters and soils, and are a source of antifungal antibiotics and acid-stable enzymes. The aim of this study is to delineate the diversity of neutrotolerant acidophilic streptomycetes in acidic soil environment, and to investigate the resources of species. Methods] 367 actinomycetes with cultural characteristics of streptomycetes were isolated from 14 acidic soil samples collected in Yunnan Province, China, using the method of DDC (dispersion and differential centrifugation) and a selective medium. The isolates were color grouped on the basis of their properties of aerial mycelium, substrate mycelium and diffusible pigments. 97 representative isolates were picked from the color groups for micromorphological observation and for the test of pH range for growth. Among the neutrotolerant acidophilic streptomycetes, 16 representatives were further selected and were subjected to a molecular systematic study based on almost complete 16S rRNA gene sequences and DNA-DNA relatedness. Results] The isolates were assigned to 12 color groups, and 80% of them were neutrotolerant acidophilic streptomycetes. The 16 representative strains formed eight distinct subclades within the genus of Streptomyces, and probably represented at least eight new genotypic species of Streptomyces. Conclusion] The neutrotolerant acidophilic streptomycetes isolated in this study were placed in eight distinct evolutionary groups, indicating the good diversity and novelty of these microorganisms in acidic soils in Yunnan Province.
%K Neutrotolerant acidophilic streptomycetes
%K 16S rRNA gene
%K Phylogenetic analysis
%K Diversity
%K Evolutionary group<br>中度嗜酸链霉菌
%K 16S
%K rRNA基因
%K 系统发育分析
%K 多样性
%K 进化群
%K 云南
%K 酸性土壤
%K 中度嗜酸链霉菌
%K Yunnan
%K Province
%K soils
%K acidic
%K isolated
%K 新物
%K 丰富多样
%K 方法筛选
%K 基因种
%K 链霉菌属
%K 分枝
%K 距离
%K 系统发育树
%K 分离菌株
%K 结果
%K 数据
%K 相关性
%K 基因组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=6BD6DFC01188CD63F5ECBE369A9B0C78&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=E158A972A605785F&sid=65C08888CCE4801E&eid=F10601728A1E9BEA&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=19