%0 Journal Article
%T Construction and analysis of a metagenomic library from Tengchong hot spring soil in Yunnan Province<br>云南腾冲热泉土壤微生物基因组文库的构建与分析
%A CAI Ying
%A CHEN Xiu-zhen
%A YANG Ke-qian
%A HUANG Li
%A DONG Zhi-yang
%A <br>蔡莹
%A 陈秀珍
%A 杨克迁
%A 黄力
%A 董志扬
%J 微生物学报
%D 2006
%I 
%X By a combination of freezing/thawing/ proteinase K-based method and SDS/high-salt/heating treatment, the mixed environmental genomic DNA was isolated directly from a hot spring soil in Tengchong, Yunnan, China. With this method, The DNA yield was up to 1 - 2 microg/g soil. After purification with the Wizard DNA clean up system (Promega, Madison, Wis), the mixed genomic DNA was partially digested with restriction enzyme Pst I. Digested DNA fragments of 3 - 8 kb were recovered from agrose gel and ligated to the pSK (+) vector. The ligation mixture was transformed into DH10B strain, resulting in the construction of a metagenomic library with about 2.5 x 10(4) clones. Restriction enzyme analysis revealed that the average insert is about 4.6 kb. Some novel sequences were identified via sequencing and gene annotation analysis of 30 clones randomly chosen from this library.
%K Culture-independent method
%K Uncultured microorganism
%K Metagenomic library<br>免培养技术
%K 未培养微生物
%K 环境基因组文库
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=65BDDAD3CFD68BDE&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=38B194292C032A66&sid=CB3428B1EFB1C133&eid=6D6BFCF0101BC091&journal_id=0001-6209&journal_name=微生物学报&referenced_num=9&reference_num=20