%0 Journal Article
%T Cloning and analysis of promoter-active fragments from Corynebacterium glutamicum 10147<br>谷氨酸棒杆菌10147基因组中启动子活性片段的克隆与分析
%A Guiming Liu
%A Zhi Zhao
%A Yingzi Zhang
%A Yu Wang
%A Jiuyuan Ding
%A <br>刘桂明
%A 赵智
%A 张英姿
%A 王宇
%A 丁久元
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] To clone promoter-active fragments from Corynebacterium glutamicum for further construction of expression vectors. Methods] Random Sau3A I digested fragments of C. glutamicum 10147 chromosome were shot-gun cloned into the promoter-probe vector pAKC6 and promoter activity of the inserted fragments was selected by chloramphenicol resistance of transformed C. glutamicum cells. Results] Thirty promoter-carrying fragments were isolated. Three C. glutamicum clones harboring pAKC6 with promoter fragments displayed chloramphenicol acetyltransferase (CAT) activity of more than 24 U/mg. The fragment F57 led to the highest CAT activity of 32.50 U/mg, even more than that produced by the promoter Ptrc, 26.33 U/mg. Conclusion] The strength of promoter on fragments F21, F54 and F57 is as strong as promoter Ptrc in C. glutamicum. These fragments can be used to construct expression vector.
%K Keywords: Corynebacterium glutamicum
%K promoter-probe vector
%K promoter
%K chloramphenicol acetyltransferase<br>关键词：谷氨酸棒杆菌，启动子探测载体，启动子，氯霉素乙酰转移酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=CC0BFB77AEBD0CBD97EEA2E99CA03351&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=DF92D298D3FF1E6E&sid=8115E88DD41C4B46&eid=1BE4748776BF02C4&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=17