%0 Journal Article
%T One-step cytometric bead array for rapid detection of Potato Virus A<br>流式微球一步法快速免疫检测马铃薯A病毒
%A Haixia Gao
%A Mingqiang Zou
%A Ling Wang
%A Jizhi Jiang
%A Shimin Tian
%A <br>高海霞
%A 邹明强
%A 王岭
%A 蒋继志
%A 田世民
%J 微生物学报
%D 2008
%I 
%X Objective] Based on immunoassay, we developed a method to detect Potato Virus A (PVA) using one-step cytometric bead array (CBA). Methods] Using fluorescent beads as carriers, we formed the complexes of bead-capture an-tibody-PVA-fluoresceinisothiocyanate (FITC) labeled detection antibody by immunoreaction of double antibody sandwich on bead surface. We measured the fluorescent signals produced from beads and from FITC with a multiparameter flow cytometer. Results] By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Conclusion] A one-step CBA method for rapid and sensitive detection of PVA is developed.
%K cytometric bead array
%K one-step
%K potato virus A
%K detection<br>流式微球技术
%K 一步法
%K 马铃薯A病毒
%K 检测
%K 流式
%K 微球
%K 一步法
%K 快速
%K 免疫检测
%K 马铃薯
%K 病毒
%K Virus
%K Potato
%K rapid
%K detection
%K array
%K ELISA
%K 微孔板
%K 检测灵敏度
%K 样品
%K 阳性
%K 交叉反应
%K 番茄
%K 花叶
%K 莴苣
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=3E452EA405CE0573FE4F67B019E63BDF&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=38B194292C032A66&sid=A7AE820C12CC9AD3&eid=05340B75C67FF664&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=14