%0 Journal Article
%T Fusion of beta1-toxin gene and beta2-toxin gene from Clostridium perfringens type C<br>C型产气荚膜梭菌β1、β2毒素基因的融合
%A XU Chong-bo
%A ZENG Jin
%A XU Chong-li
%A WANG Yu-jiong
%A <br>许崇波
%A 曾瑾
%A 许崇利
%A 王玉炯
%J 微生物学报
%D 2005
%I 
%X Betal-toxin and beta2-toxin genes from chromosomal DNA of Clostridium perfringens type C were amplified by PCR, PCR products were cleaved with restriction endonucleases and recovered. The recombinant plasmid pETXB1-2 containing beta1-beta2 fusion genes was constructed by recombinant technique and then transformed into Escherichia coli BL21 (DE3). The beta1-beta2 fusion proteins were expressed in recombinant strain BL21 (DE3) (pETXB1-2), and the expression level of the beta1-beta2 fusion proteins was about 15.36% of total cellular protein by SDS-PAGE and thin-layer gel scanning analysis. More importantly, immunization in a mouse model with crude preparation containing the fusion protein inclusion bodies or inactivated recombinant strain induced protection against at least 1 MLD of the toxin from Clostridium perfringens type C. Hence the fusion proteins possess a good immunogenicity. The constructed recombinant strain BL21 (DE3) (pETXB1-2) can be used as a candidate of vaccine strain.
%K Clostridium perfringens type C
%K Beta1-toxin gene
%K Beta2-toxin gene
%K Gene fusion
%K Fusion protein
%K Gene expression<br>产气荚膜梭菌
%K β1毒素
%K β2毒素
%K 融合基因
%K 基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=84C7A157590B00DD&yid=2DD7160C83D0ACED&vid=94E7F66E6C42FA23&iid=0B39A22176CE99FB&sid=EF27C460877D3C9F&eid=3D9746C06EC12B45&journal_id=0001-6209&journal_name=微生物学报&referenced_num=6&reference_num=7