%0 Journal Article
%T Influence of Acid Adaptation on Membrane Fatty Acid Profile and Expression of Membrane proteins in Lactococcus lactis<br>酸适应对一株乳酸乳球菌膜脂肪酸组成和膜蛋白表达的影响
%A Huailong Liu
%A Xiangchen Meng
%A <br>刘怀龙
%A 孟祥晨
%J 微生物学报
%D 2008
%I 
%X Abstract: Objective] Lactococcus lactis with high acid adaptive capacity was screened, and the influence of acid adap-tation on membrane fatty acid profile and expression of membrane proteins were studied. Methods] Lactococcus lactis KLDS4.0312 with high acid adaptive capacity was screened from 12 lactic acid bacteria and 8 Bifidobacterium. The change of the fatty acid profile in cell membrane of this strain was analyzed by gas chromatography-mass spectrometry, and two dimensional polyacrylamide gel electrophoresis method was used to analyze the differential expression of mem-brane proteins during the acid adaptation. Results] After acid adaptation, Lactococcus lactis KLDS4.0312 increased the proportion of unsaturated fatty acids from 30.77% to 42.93% in its membrane lipids with a concomitant decrease in satu-rated fatty acids from 69.23% to 57.07%, and a new long-chained, mono-unsaturated fatty acid C19:1-n6 was induced. Compared with the non-adapted sample, there were at least 65 distinct protein points in the adapted sample, where 43 proteins were up-regulated and 22 proteins were down-regulated. But the acid adaptive capacity of Lactococcus lactis KLDS4.0312 was eliminated by adding chloramphenicol which can restrain the synthesis of new proteins. Conclusion] These results showed that change in the membrane fatty acid profile by acid adaptation in Lactococcus lactis KLDS4.0312.
%K Keywords: acid adaptation
%K Lactococcus lactis
%K fatty acids
%K shock protein
%K survival rate<br>酸适应
%K 乳酸乳球菌
%K 脂肪酸
%K 应激蛋白
%K 存活率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=8CA388ECAF4D83F795A4293136640DA2&yid=67289AFF6305E306&vid=B6DA1AC076E37400&iid=708DD6B15D2464E8&sid=AA170179913DEBCD&eid=4D36A7D6CAFA227E&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=15