%0 Journal Article
%T The Defective Potassium Uptake by TRK1 and TRK2 Mutant of Saccharomyces cerevisiae Using Homologous Recombination<br>酿酒酵母TRK1和TRK2钾吸收缺陷型菌株的构建
%A GUO Zhao-Kui
%A YANG Qian
%A YAN Pei-Qiang
%A WAN Xiu-Qing
%A YAO Quan-Hong
%A <br>郭兆奎
%A 杨 谦
%A 颜培强
%A 万秀清
%A 姚泉洪
%J 微生物学报
%D 2008
%I 
%X In order to perform the regulation and functional study and the screening of potassium uptake genes, the high affinity K+ uptake genes TRK1 and TRK2 in Saccharomyces cerevisiae were replaced with URA3 and HIS3 alternately using the methods of homologous recombination. URA3 and HIS3 of S. Cerevisiae were used as marker gene, the defective K+ uptake TRK1 and TRK2 mutant of S. Cerevisiae were selected in the basic medium without Histidine and Uracil.
%K Saccharomyces cerevisiae
%K TRK1 mutant
%K TRK2 mutant
%K Homologous recombination<br>酵母
%K TRK1、TRK2吸钾基因缺陷型
%K 同源重组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=3831199FADFD75C810587121F08816F8&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=0B39A22176CE99FB&sid=4AD6A901BD736716&eid=089D6A5945E87569&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0