%0 Journal Article
%T Progress in biosynthesis of epsilon-poly-L-lysine and genetic transformation for epsilon-poly-L-lysine producer--a review<br>ε-聚赖氨酸生物合成及其产生菌遗传转化研究进展
%A Qingping Wu
%A Shengrong Liu
%A Jumei Zhang
%A <br>吴清平
%A 刘盛荣
%A 张菊梅
%J 微生物学报
%D 2011
%I 
%X Epsilon-Poly-L-lysine (epsilon-PL) is a polymer with strong antimicrobial activity and consists of 25-35 L-lysine monomers linked via epsilon-amino-alpha-carboxyl peptide bond. This polymer is one of only two homo-poly (amino acid) polymers known in nature. To date, the biosynthesis mechanism of epsilon-PL was remained unclear. Recently, epsilon-PL synthetase was identified as a nonribosomal peptide synthetase and it utilized L-lysine to synthesize epsilon-PLs of various chain length by iteratively condensing reaction, which was similar to type I polyketide synthase and was not determined by epsilon-PL-degrading enzyme. Meanwhile, the shutter vectors pLAE001 and pLAE003, special for epsilon-PL producer have been constructed, which is significant for studying epsilon-PL biosynthesis. In this review, the biosynthesis of epsilon-PL and the genetic transformation were introduced. Also, the study in our group was presented and the views related to e-PL study were raised. Finally, the prospect of application of combinational biosynthesis in epsilon-PL-producing strain improvement was also discussed considering epsilon-PL synthetase is a nonribosomal peptide synthetase containing several modules.
%K ε-poly-L-lysine
%K biosynthesis
%K genetic transformation system
%K combinational biosynthesis<br>ε-聚赖氨酸
%K 生物合成
%K 遗传转化体系
%K 组合生物合成
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=B4DA16C860E8C53F351F8C2AA08EC4E0&yid=9377ED8094509821&vid=987EDA49D8A7A635&iid=B31275AF3241DB2D&sid=5A027C8E6C570AAB&eid=94655B9881133A28&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=41