%0 Journal Article
%T Identification and analysis of an actinomycete strain suppressing Clavibacter michiganensis subsp. Michiganensis<br>番茄溃疡病菌拮抗菌株Z-L-22的鉴定及其活性物质
%A Yan Zhang
%A Weihong Zhang
%A Songhong Wang
%A Yaning Li
%A Zhiquan Zhao
%A Daqun Liu
%A Wenxiang Yang
%A <br>张艳
%A 张维宏
%A 王松红
%A 李亚宁
%A 赵志泉
%A 刘大群
%A 杨文香
%J 微生物学报
%D 2009
%I 
%X Abstract: Objective] To identify and analyze bioactive compounds of an actinomycete strain Z-L-22 suppressing Clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato. Methods] Morphological, biological and biochemical characterization, chemotaxonomy analysis and 16S rDNA sequences homology analysis were performed to identify the strain Z-L-22. Bioactive compounds were separated and retrieved by thin layer chromatography. Paper chromatography and confirmation tests were used to identify the antibiotic. PCR was carried out using the primers targeted to synthetase of the antibiotic. Results] Strain Z-L-22 belonged to Streptomyces sp. and was similar to Streptomyces setonii. Two main bioactive components were isolated by thin layer chromatography, which were all identified as actinomycin. New actinomycin synthetase gene was cloned using the primers designed from actinomycin synthetase conserve domain. Conclusion] Strain Z-L-22 was classified as Streptomyces setonii. Actinomycin produced by Streptomyces setonii was first reported.
%K Key words: strain Z-L-22
%K Streptomyces setonii
%K bacterial canker of tomato
%K actinomycin<br>关键词：Z-L-22菌株
%K 西唐链霉菌
%K 番茄溃疡病
%K 放线菌素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=C81364104C88BFA47068B62D963041FE&yid=DE12191FBD62783C&vid=2A3781E88AB1776F&iid=DF92D298D3FF1E6E&sid=BF6EDE6C10074464&eid=2DEC3FE1EFC628C2&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=20