%0 Journal Article
%T Improving heat and pH stability of nisin by site-directed mutagenesis<br>通过定点突变提高乳链菌肽对热及pH的稳定性
%A Yao Lu
%A Like Jiang
%A Meiling Chen
%A Liandong Huan
%A Jin Zhong
%A <br>路遥
%A 蒋立科
%A 陈美玲
%A 还连栋
%A 钟瑾
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] The aim of this study was to optimize the property of nisin through altering its specific amino acid by site-directed mutagenesis method. Methods] On the basis of M21K nisinZ, a former reported nisinZ mutant that exhibited antimicrobial activity against Gram-negative bacteria, the 29th amino acid of it was mutated from serine to lysine. The mutant M21K/S29K nisZ gene was cloned into vector pMG36e and the recombinant plasmid was introduced into Lacotococcus lactis NZ9800. The resulting M21K/S29K nisinZ was then isolated and purified, and its antibacterial activity, antibacterial spectrum and stability were analyzed and compared to those of M21K nisinZ and nisinZ. Results] Compared with wild-type nisinZ and M21K nisinZ, the M21K/S29K nisinZ displayed reduced antimicrobial activity, but showed significantly increased stabilities to heat and pH stress. Moreover, M21K/S29K nisinZ also exhibited antimicrobial activity against Gram-negative bacteria as M21K nisinZ did. Conclusion] By changing the 29th amino acid of nisin, we can optimize the property of nisin, especially its stability to heat and pH stress.
%K 关键词：乳链菌肽
%K 稳定性
%K 温度
%K pH
%K 定点突变
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=7D8F631775E873F33A523C14972A0371&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=708DD6B15D2464E8&sid=AB07CD602348A2CC&eid=BFA4330C9764AE1A&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0