%0 Journal Article
%T Genetic diversity and phylogeny of soybean rhizobia isolated from the regions of Loess Plateau in China<br>黄土高原地区大豆根瘤菌的遗传多样性和系统发育
%A Hongxia Zhang
%A Ruihua Feng
%A Jun Li
%A Dawei Guan
%A Fengming Cao
%A <br>张红侠
%A 冯瑞华
%A 李俊
%A 关大伟
%A 曹凤明
%J 微生物学报
%D 2010
%I 
%X Abstract: Objective] We investigated the genetic diversity and phylogeny of soybean rhizobia isolated from the regions of Loess Plateau in China. Methods] We analyzed 130 soybean rhizobia isolated from 15 regions in 4 provinces of Loess Plateau through BOX-PCR, 16S rDNA PCR- RFLP, 16S-23S IGS PCR-RFLP and 16S rRNA gene sequencing. Results] BOX-PCR, 16S rDNA PCR- RFLP and 16S-23S IGS PCR-RFLP were in good agreement with the results which showed that all strains tested ascribed to two groups: the genus of Sinorhizobium and Bradyrhizobium phylogenetically. The analysis of 16S rRNA gene of 5 representative strains indicated that they were related to type strains S. fredii, B. japonicum and B. liaoningense, homology coefficient with type strains was 100% respectively. Conclusion] Soybean rhizobia isolated from the regions of Loess Plateau in China showed rich genetic diversity. S. fredii was the dominant species. Bradyrhizobium accounted for 10% of the strains tested only, of which, two strains were B. liaoningense.
%K Keywords: Soybean rhizobia
%K BOX-PCR
%K 16S rDNA PCR-RFLP
%K 16S-23S rDNA IGS PCR-RFLP
%K genetic diversity
%K phylogeny<br>关键词：大豆根瘤菌
%K BOX-PCR
%K 16S
%K rDNA
%K PCR-RFLP
%K 16S-23S
%K rDNA
%K IGS
%K PCR-
%K RFLP
%K 遗传多样性
%K 系统发育
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=994B5DA2F28173D4F3AC45494D4CEFAE&yid=140ECF96957D60B2&vid=771152D1ADC1C0EB&iid=708DD6B15D2464E8&sid=819029E276C005ED&eid=B2A9EA001599F2ED&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0