%0 Journal Article
%T Isolation methods and diversity of culturable fecal actinobacteria associated with Panthera tigris tigris in Yunnan Safari Park<br>孟加拉虎粪便放线菌的分离及其多样性
%A Yanru Cao
%A Yi Jiang
%A Youlong Li
%A Xiu Chen
%A Rongxian Jin
%A Wenxiang He
%A <br>曹艳茹
%A 姜怡
%A 李有龙
%A 陈秀
%A 靳荣线
%A 和文祥
%J 微生物学报
%D 2012
%I 
%X Objective] We studied the isolation methods and diversity of culturable fecal actinobacteria associated with Panthera tigris tigris by using culture-dependent approaches.Methods] Fresh fecal samples of healthy Panthera tigris tigris were collected from Yunnan Safari Park.Pretreatment of the samples,isolation media and inhibitors were tested for actinobacteria isolation.16S rRNA genes of actinobacteria were sequenced and subjected to phylogenetic analysis.Results] The abundance of culturable actinobacteria was 1.10×108 cfu/g colony forming units(CFU) per gram of feces(wet weight).We obtained 110 purified cultural actinobacterium strains.The analysis based on 16S rRNA gene sequences showed that these strains were distributed in 10 different families and 12 genera of actinobacteria at least,and most of them were non-filamentous,such as Arthrobacter,Dietzia,Kocuria,Corynebacterium and Microbacterium.Streptomyces was the mainly classical filamentous actinobacteria,and up to 64% of total.Conclusion] Drying and heating up the fecal samples can greatly increase the rate of the actinobacteria.Many kinds of inhibitors and chemical defined media are suitable for isolation of fecal actinobacteria.The culturable actinobacteria are abundant in Panthera tigris tigris feces.Our study found an effective method to isolate animals’ fecal actinobacteria and it’s useful for studying and exploiting animals’ fecal actinobacteria.
%K Panthera tigris tigris
%K fecal actinobacteria
%K isolation
%K 16S rRNA gene
%K diversity<br>孟加拉虎
%K 粪便放线菌
%K 分离方法
%K 16S
%K rRNA基因
%K 多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=8324B84C7395C6562485DC06D0F42726&yid=99E9153A83D4CB11&vid=286FB2D22CF8D013&iid=DF92D298D3FF1E6E&sid=43AADF4B53A8BF6F&eid=8A03DD854A27B60D&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=0