%0 Journal Article
%T Screening of microorganism producing polyunsaturated fatty acids with sugarcane molasses<br>甘蔗糖蜜发酵生产多不饱和脂肪酸的菌种筛选
%A LI Nan
%A DENG Zhi-nian
%A QIN Yong-ling
%A LING Min
%A LIANG Zhi-qun
%A <br>李楠
%A 邓智年
%A 覃拥灵
%A 凌敏
%A 梁智群
%J 微生物学报
%D 2006
%I 
%X A strain name LB1 producing polyunsaturated fatty acids with sugarcane molasses was screened form soil samples by the methods of Sudan Black B stain and the determination of PUFAs' iodine value. Based on the grass morphological and physiological characteristics and the sequence similarity of ribosomal DNA-ITS, the strain was identified as Mucor recurvus sp.. The results of L64 (4(3)) orthogonal experiments indicated when Mucor recurvus was cultivated at 28 degrees C for 5 days with 160 r/min, the yields of PUFAs with 10(0) BX sugarcane molasses (pH 6.0) were up to 80.49% content of total fatty acids. The total lipids content was 57.08% of fermentation biomass, which were composed of 15.42% Oleic acid, 14.38% Linolenic, 23.55% gamma-Linolenic, 3.06% alpha-Linolenic, 9.87% Arachidonic acid, 8.14% Eicosapentaenoic acid, 6.07% Docosahexaenoic acid, etc.
%K Mucor sp
%K LB1
%K Polyunsaturated fatty acids
%K Sugarcane molasses
%K Cultivation condition
%K Identification<br>毛霉LB1
%K 多不饱和脂肪酸
%K 甘蔗糖蜜
%K 发酵条件
%K 鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=2A4F86D94FF54751&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=E158A972A605785F&sid=385E3C2062167B88&eid=CF0706A3E35031F6&journal_id=0001-6209&journal_name=微生物学报&referenced_num=1&reference_num=22