%0 Journal Article
%T Ssh10b2 differs from its paralogue Ssh10b in cellular abundance and the ability to constrain DNA supercoils<br>Ssh10b2与其同源蛋白Ssh10b在细胞含量及固定DNA超螺旋的能力上存在明显差异
%A GUO Rong
%A XUE Hong
%A HUO Xiao-feng
%A XU Dong-yi
%A HU Jin-chuan
%A <br>郭荣
%A 薛宏
%A 霍笑风
%A 徐冬一
%A 胡晋川
%J 微生物学报
%D 2006
%I 
%X The ssh10b and ssh10b2 genes, a pair of distantly related paralogues in Sulfolobus shibatae, encode members of the Sac10b DNA binding protein family in thermophilic archaea. It has been shown previously that Ssh10b exists in abundance in S. shibatae and is capable of constraining negative DNA supercoils, properties that are consistent with a speculated architectural role for the protein in chromosomaforganization. In this study, the ssh10b2 gene was cloned and expressed in Escherichia coli, and the recombinant Ssh10b2 protein was purified to apparent homogeneity. Immunoblotting analysis using a specific anti - Ssh10b2 antibody showed that ssh10b2 was expressed in S. shibatae, but the cellular level of Ssh10b2 was only - 10% of that of Ssh10b. Recombinant Ssh10b2 was capable of interacting with both double-stranded and single-stranded DNA. The affinity of the protein for double-stranded DNA was higher than that reported for Ssh10b. The Ssh10b2 and Ssh10b proteins appeared to generate similar gel shift patterns on duplex DNA fragments. However, unlike Ssh10b, Ssh10b2 was unable to constrain DNA supercoils. These data suggest that Ssh10b2 does not serve as a general architectural factor in DNA compaction and organization in S. shibatae.
%K Ssh10b2
%K Sac10b family
%K Sulfolobus shibatae
%K Cellular abundance
%K DNA binding
%K DNA supercoiling<br>极端嗜热古菌
%K 芝田硫化叶菌
%K Sac10b家族
%K Ssh10b2
%K DNA超螺旋
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=ACD29C94DC21CDED&yid=37904DC365DD7266&vid=D997634CFE9B6321&iid=0B39A22176CE99FB&sid=892C6E385D640C1E&eid=302FBD968ED44801C3EED05B1A476849&journal_id=0001-6209&journal_name=微生物学报&referenced_num=0&reference_num=24