%0 Journal Article
%T Effects of the Radix Ranuncoli Ternati extracts on Mycobacterium tuberculosis proteome profiling revealed by 2D electrophoresis<br>猫爪草提取物对临床分离结核分枝杆菌蛋白质组表达的影响
%A HE Ying
%A YUE Jun
%A HU Chang-hua
%A WANG Hong-hai
%A XIE Jian-ping
%A TANG Cui
%A WEI Xiu-li
%A LIU Xue-mei
%A SONG Jie
%A <br>何颖
%A 乐军
%A 胡昌华
%A 王洪海
%A 谢建平
%J 微生物学报
%D 2005
%I 
%X Radix Ranuncoli Ternati is clinically effective traditional Chinese medicine for multidrug resistant tuberculosis. Its active components and mechanism of action remain unsolved. Two dimensional gel electrophoresis(2-DE)was employed to address this problem. Globlal proteome of Mycobacterium tuberculosis untreated and treated with Radix Ranuncoli Ternati were compared, and 22 protein spots were found to be expressed differentially. 3 protein spots which remarkably decreased in Mycobacterium tuberculosis treated with Radix Ranuncoli Ternati were subjected to matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS) analysis. The data obtained from peptide mass finger printing were used for database search. The 3 protein spots in gel were identified as cysA2(thiosulfate sulfurtransferase),tsf(elongation factor EF-Ts)and hspX(heat shock protein X).These data provide insights into the changed global protein patterns of Mycobacterium tuberculosis treated with Radix Ranuncoli Ternati and may prove useful for further study in the mechanism in how Radix Ranuncoli Ternati influence the life of Mycobacterium tuberculosis.The differentially expressed proteins may be potential novel antituberculosis drug targets.
%K Radix Ranuncoli Ternati
%K Mycobacterium tuberculosis
%K Proteome
%K Two dimensional gel electrophoresis(2-DE)
%K Differential expression<br>猫爪草
%K 结核分枝杆菌
%K 蛋白质组
%K 双向电泳
%K 差异表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A3C6BA55AB623B90FA9104CFFC826F3C&aid=FF04D0C87C1C46A0&yid=2DD7160C83D0ACED&vid=94E7F66E6C42FA23&iid=B31275AF3241DB2D&sid=2DEC3FE1EFC628C2&eid=7E7F5B01D43BD73F&journal_id=0001-6209&journal_name=微生物学报&referenced_num=5&reference_num=20